Application
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using GEFT antibody at 1:250 dilution.| Product Name | GEFT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GEFT (Rho guanine nucleotide exchange factor (GEF) 25)The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange (Souchet et al., 2002 [PubMed 11861769]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GEFT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (85%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GEFT antibody, p63RhoGEF antibody, ARHGEF25 antibody, rac/Cdc42/Rho exchange factor GEFT antibody, rho guanine nucleotide exchange factor 25 antibody, guanine nucleotide exchange factor GEFT antibody, rhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT |
|---|---|
| Molecular Weight(MW) | 64 kDa (note) (3) |
| Cellular Localization | Cytoplasm , myofibril , sarcomere |
Application
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using GEFT antibody at 1:250 dilution.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
