Application
WB analysis of extracts from various cell lines using Gemin 3 antibody.Application
ICC/IF analysis of HeLa cells using Gemin 3 antibody (green) and DAPI (blue).| Product Name | Gemin 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Gemin 3DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human DDX20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Recommended Starting Dilutions: For WB: Use at a dilution of 1:500-1:2000. For IHC: Use at a dilution of 1:50-1:200. For ICC/IF: Use at a dilution of 1:10-1:100. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DP103 , GEMIN3 , DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 , DDX20 |
|---|---|
| Target | Gemin 3 |
| Molecular Weight(MW) | 92 kDa (note) |
Application
WB analysis of extracts from various cell lines using Gemin 3 antibody.
Application
ICC/IF analysis of HeLa cells using Gemin 3 antibody (green) and DAPI (blue).| Application Notes | Recommended Starting Dilutions: For WB: Use at a dilution of 1:500-1:2000. For IHC: Use at a dilution of 1:50-1:200. For ICC/IF: Use at a dilution of 1:10-1:100. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
