Application
Gemin 5 antibody detects Gemin 5 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Gemin 5 protein stained by Gemin 5 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunoprecipitation of Gemin 5 protein from HepG2 whole cell extracts using 5 μg of Gemin 5 antibody . Western blot analysis was performed using Gemin 5 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Gemin 5 antibody detects Gemin 5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gemin 5 antibody diluted at a dilution of 1:500.| Product Name | Gemin 5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Gemin 5 (gem (nuclear organelle) associated protein 5)GEMIN5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), and GEMIN4 (MIM 606969).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Gemin 5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | gem (nuclear organelle) associated protein 5 GEMIN-5 GEMIN5 |
|---|---|
| Target | Gemin 5 |
| Molecular Weight(MW) | 169 kDa (note) (2) |
| Cellular Localization | Nucleus , nucleoplasm , gem , Cytoplasm |
Application
Gemin 5 antibody detects Gemin 5 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Gemin 5 protein stained by Gemin 5 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunoprecipitation of Gemin 5 protein from HepG2 whole cell extracts using 5 μg of Gemin 5 antibody . Western blot analysis was performed using Gemin 5 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Gemin 5 antibody detects Gemin 5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gemin 5 antibody diluted at a dilution of 1:500.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
