Application
WB analysis of 293 lysate using Geminin antibody in the abscence (lane 1) or prescence (lane 2) of blocking peptide.| Product Name | Geminin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to GemininGeminin, inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. It has a destruction box sequence (RRTLKVIQP) and is ubiquitinated by the anaphase-promoting complex in vitro. Geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading. |
| Immunogen | Synthetic peptide: SSENKNLGGVTQES-C conjugated to KLH, corresponding to amino acids 72-85 of Human Geminin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: Use at a dilution of 1/500. Detects a band of approximately 33 kDa (predicted molecular weight: 23.5 kDa). Can be blocked with GTX12408 Geminin peptide . Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | O75496 Geminin RP3369A17.3 602842 Gem 51053 GMNN RP3-369A17.3 |
|---|---|
| Target | Geminin |
| Tissue Specificity | This antibody detects a band representing Geminin by Western blot at 33kD on 293T cell lysate, this band can be blocked by the immunising peptide. It also detects a band at about 45kD that is not blocked by the immunising peptide and is thought to be non-specific. |
Application
WB analysis of 293 lysate using Geminin antibody in the abscence (lane 1) or prescence (lane 2) of blocking peptide.| Application Notes | WB: Use at a dilution of 1/500. Detects a band of approximately 33 kDa (predicted molecular weight: 23.5 kDa). Can be blocked with GTX12408 Geminin peptide . Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: 1% BSA, PBS. pH 7.4 |
