Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using GERP antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000Application
GERP antibody detects GERP protein by western blot analysis. Non-transfected (-) and -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GERP antibody diluted by 1:5000.| Product Name | GERP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GERP (tripartite motif containing 8)The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to nuclear bodies. Its structure is similar to some tumor suppressor proteins and its gene maps to a locus thought to contain tumor suppressor genes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GERP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Cat, Chicken, Rhesus Monkey, BovineMouse (98%), Rat (98%), Xenopus laevis (91%), Cat (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GERP antibody RNF27 antibody TRIM8 antibody probable E3 ubiquitin-protein ligase TRIM8 antibody tripartite motif-containing protein 8 antibody ring finger protein 27 antibody glioblastoma-expressed RING finger protein antibody tripartite motif-conta |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using GERP antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000
Application
GERP antibody detects GERP protein by western blot analysis. Non-transfected (-) and -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GERP antibody diluted by 1:5000.| Positive Control | HepG2 , 3XFlag-human TRIM8-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
