Application
GFAP antibodies detects GFAP proteins on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Green: GFAP antibody diluted at 1:1000. Red: Sox2 antibody [GT1352] diluted at 1:500.Application
GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GFAP protein stained by GFAP antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: GFAP antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) . Blue: Nuclear staining with Hoechst 33342.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GFAP antibody diluted at 1:500.| Product Name | GFAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GFAP (glial fibrillary acidic protein)This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (94%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ45472 antibody GFAP antibody glial fibrillary acidic protein antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
GFAP antibodies detects GFAP proteins on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Green: GFAP antibody diluted at 1:1000. Red: Sox2 antibody [GT1352] diluted at 1:500.
Application
GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GFAP protein stained by GFAP antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: GFAP antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) . Blue: Nuclear staining with Hoechst 33342.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GFAP antibody diluted at 1:500.| Positive Control | U87-MG , SK-N-SH , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
