Application
GGA3 antibody [C3], C-term detects GGA3 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GGA3 antibody [C3], C-term diluted at 1:500.Application
GGA3 antibody [C3], C-term detects GGA3 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GGA3 protein stained by GGA3 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using GGA3 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:2000| Product Name | GGA3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GGA3 (golgi-associated, gamma adaptin ear containing, ARF binding protein 3)This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GGA3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Chicken, Rat, Xenopus TropicalisRat (92%), Xenopus Tropicalis (83%), Chicken (83%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0154 antibody GGA3 antibody ADP-ribosylation factor-binding protein GGA3 antibody Golgi-localized gamma ear-containing ARF-binding protein 3 antibody golgi associated gamma adaptin ear containing ARF binding protein 3 antibody golgi-associated |
|---|---|
| Molecular Weight(MW) | 78 kDa (note) (5) |
| Cellular Localization | Golgi apparatus , trans-Golgi network membrane; Peripheral membrane protein , Endosome membrane; Peripheral membrane protein |
Application
GGA3 antibody [C3], C-term detects GGA3 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GGA3 antibody [C3], C-term diluted at 1:500.
Application
GGA3 antibody [C3], C-term detects GGA3 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GGA3 protein stained by GGA3 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using GGA3 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:2000| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
