Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 7.5% SDS PAGE diluted at 1:3000Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using GGCX antibody at 1:500 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GGCX antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | GGCX antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GGCX (gamma-glutamyl carboxylase)This gene encodes an enzyme which catalyzes the posttranslational modification of vitamin K-dependent protein. Many of these vitamin K-dependent proteins are involved in coagulation so the function of the encoded enzyme is essential for hemostasis. Mutations in this gene are associated with vitamin K-dependent coagulation defect and PXE-like disorder with multiple coagulation factor deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GGCX. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Sheep, Rhesus Monkey, BovineMouse (95%), Rat (94%), Sheep (96%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ26629 antibody VKCFD1 antibody GGCX antibody peptidyl-glutamate 4-carboxylase antibody vitamin K-dependent gamma-carboxylase antibody gamma-glutamyl carboxylase antibody |
|---|---|
| Molecular Weight(MW) | 88 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein |
Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 7.5% SDS PAGE diluted at 1:3000
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using GGCX antibody at 1:500 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GGCX antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
