Application
Sample(30 µg of whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GGT1 antibody detects GGT1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE GGT1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using GGT1 antibody at 1:100 dilution.| Product Name | GGT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GGT1 (gamma-glutamyltransferase 1)The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GGT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, BovineRat (80%), Pig (85%), Bovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD224 antibody D22S672 antibody D22S732 antibody GGT antibody GGT 1 antibody GTG antibody MGC96892 antibody MGC96904 antibody MGC96963 antibody GGT1 antibody gamma-glutamyl transpeptidase antibody gamma-glutamyltranspeptidase 1 antibody glutamy |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (6) |
| Cellular Localization | Membrane; Single-pass type II membrane protein |
Application
Sample(30 µg of whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GGT1 antibody detects GGT1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE GGT1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using GGT1 antibody at 1:100 dilution.| Positive Control | Molt-4 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
