Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using GHS-R1 antibody at 1:100 dilution.Application
GHS-R1 antibody [N1], N-term detects GHSR protein by western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract 10% SDS-PAGE GHS-R1 antibody [N1], N-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GHS-R1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GHS-R1 (growth hormone secretagogue receptor)This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GHS-R1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | PigPig (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GHSR antibody growth hormone secretagogue receptor type 1 antibody GH-releasing peptide receptor antibody ghrelin receptor antibody GHRP antibody GHS-R antibody growth hormone secretagogue receptor antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (2) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using GHS-R1 antibody at 1:100 dilution.
Application
GHS-R1 antibody [N1], N-term detects GHSR protein by western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract 10% SDS-PAGE GHS-R1 antibody [N1], N-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NT2D1 , SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
