Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody diluted at 1:1000.Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody diluted at 1:1000.| Product Name | GIT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GIT1 (G protein-coupled receptor kinase-interactor 1)GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of mouse GIT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | MouseRat |
| Predicted Reactivity | HumanHuman (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARF GAP GIT1 antibody Cat1 antibody G protein-coupled receptor kinase-interactor 1 antibody p95Cat antibody |
|---|---|
| Target | GIT1 |
| Molecular Weight(MW) | 85 kDa (note) |
| Cellular Localization | Cytoplasm (By similarity) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody diluted at 1:1000.
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody diluted at 1:1000.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
