Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using GIT1 antibody at 1:500 dilution.Application
GIT1 antibody [N3C2], Internal detects GIT1 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE GIT1 antibody [N3C2], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human mixed ovarian cancer, using GIT1 antibody at 1:100 dilution.Application
Sample(30 µg of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GIT1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GIT1 (G protein-coupled receptor kinase interacting ArfGAP 1) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GIT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, BovineMouse (95%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GIT1 antibody CAT-1 antibody CAT1 antibody cool-associated and tyrosine-phosphorylated protein 1 antibody G protein-coupled receptor kinase-interactor 1 antibody G protein-coupled receptor kinase interactor 1 antibody GRK-interacting protein 1 antibo |
|---|---|
| Molecular Weight(MW) | 84 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using GIT1 antibody at 1:500 dilution.
Application
GIT1 antibody [N3C2], Internal detects GIT1 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE GIT1 antibody [N3C2], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human mixed ovarian cancer, using GIT1 antibody at 1:100 dilution.
Application
Sample(30 µg of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 , Rat2 , HUVEC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
