Application
WB ImageRat liver extract was separated on SDS-PAGE and probed with APA to GJB1 (265-279) (RB) . The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate. Antibody concentration 1/1,200| Product Name | GJB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Connexin 32 / GJB1Gap junctions are aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control and developmental regulation. The gap junction channels allow intercellular exchange of ions, nucleotides and small molecules between adjacent cells. Unlike other membrane channels, intercellular channels span two plasma membranes and require the contribution of hemichannels, called connexons, from both participating cells. These channels are permeate to molecules as large as 1 kD, and they have been detected in virtually every cell type in mammals, except mature skeletal muscle, spermatozoa and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins, which are termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins ranging between molecular weights of 26 and 70 kD. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group to which Cx26, Cx30, Cx31, Cx31.1 and Cx32 belong, and the other termed class II or alpha group, represented by Cx33, Cx37, Cx40, Cx43 and Cx46. |
| Immunogen | Synthetic peptide: KRSPGTGAGLAEKSDR conjugated to KLH, corresponding to amino acids 265-279 of GJB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FWB |
| IHC-Fr: Use at a dilution of 1/400. WB: Use at a dilution of 1/600. Detects a band of approximately 27 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CX32 CMTX CMTX1 2705 302800 P08034 GJB1 |
|---|---|
| Target | GJB1 |
Application
WB ImageRat liver extract was separated on SDS-PAGE and probed with APA to GJB1 (265-279) (RB) . The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate. Antibody concentration 1/1,200| Application Notes | IHC-Fr: Use at a dilution of 1/400. WB: Use at a dilution of 1/600. Detects a band of approximately 27 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
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| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS (pH7.4) |
