Application
Mouse brain extract was separated on SDS-PAGE and probed with Monoclonal AB Connexin 32 (MO) Clone: CXN-32 . The antibody was developed with Al-Hh APA to Mouse IgG (Fab)(Gt) and a BCIP/NBT substrate. Antibody dilution 1/1,000.| Product Name | GJB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CXN - 32] to GJB1Gap junctions are aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control and developmental regulation. The gap junction channels allow intercellular exchange of ions, nucleotides and small molecules between adjacent cells. Unlike other membrane channels, intercellular channels span two plasma membranes and require the contribution of hemichannels, called connexons, from both participating cells. These channels are permeate to molecules as large as 1 kD, and they have been detected in virtually every cell type in mammals, except mature skeletal muscle, spermatozoa and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins, which are termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins ranging between molecular weights of 26 and 70 kD. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group to which Cx26, Cx30, Cx31, Cx31.1 and Cx32 belong, and the other termed class II or alpha group, represented by Cx33, Cx37, Cx40, Cx43 and Cx46. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 105-123 of GJB1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CXN-32 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FWB |
| ELISA: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 27 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CX32 CMTX CMTX1 2705 302800 P08034 GJB1 |
|---|---|
| Target | GJB1 |
Application
Mouse brain extract was separated on SDS-PAGE and probed with Monoclonal AB Connexin 32 (MO) Clone: CXN-32 . The antibody was developed with Al-Hh APA to Mouse IgG (Fab)(Gt) and a BCIP/NBT substrate. Antibody dilution 1/1,000.| Positive Control | Tested whith whole brain extract |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 27 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Raw Ascites |
