Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Gli1 antibody detects Gli1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gli1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Gli1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Gli1 (GLI-Kruppel family member GLI1)May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Gli1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (100%), Rat (100%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | zinc finger protein GLI1 antibody glioma-associated oncogene homolog antibody zinc finger protein 5 antibody AV235269 antibody Zfp-5 antibody Zfp5 antibody Gli1 antibody GLI-Kruppel family member GLI1 antibody |
|---|---|
| Molecular Weight(MW) | 119 kDa (note) |
| Cellular Localization | Cytoplasm (By similarity) , Nucleus (By similarity) |
Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Gli1 antibody detects Gli1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gli1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | PC-3 , U87-MG , SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
