Application
Sample (30 µg of whole cell lysate) A: Jurkat 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3 antibody at 1:500 dilution.Application
GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. GLI3 antibody diluted at 1:250.Application
Sample (20 µg of whole cell lysate) A: mouse ESC 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GLI3 antibody detects GLI3 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 5% SDS-PAGE GLI3 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GLI3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GLI3 (GLI family zinc finger 3)This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GLI3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, ChimpanzeeXenopus laevis (87%), Chicken (92%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACLS antibody GCPS antibody PAP-A antibody PAPA antibody PAPA1 antibody PAPB antibody PHS antibody PPDIV antibody GLI3 antibody oncogene GLI3 antibody zinc finger protein GLI3 antibody GLI-Kruppel family member GLI3 antibody glioma-associated o |
|---|---|
| Molecular Weight(MW) | 170 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 µg of whole cell lysate) A: Jurkat 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3 antibody at 1:500 dilution.
Application
GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. GLI3 antibody diluted at 1:250.
Application
Sample (20 µg of whole cell lysate) A: mouse ESC 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GLI3 antibody detects GLI3 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 5% SDS-PAGE GLI3 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , mouse ESC , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
