Application
WB analysis of ACHN cells using GLS antibody at 0.2-1μg/ml.Application
WB analysis of rat kidney cortex, kidney proximal tubules from cortex, rat brain tissue, and pig LLCPK1-F cells using GLS antibody at 1:2000. Lane 5 : rat kidney cortex Lane 6 : rat kidney proximal tubules from cortex Lane 7 : pig LLCPK1-F cells Lane 8 : rat brain| Product Name | GLS antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GLSThis gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| Immunogen | A synthetic peptide corresponding to a C-terminal region of Human GLS |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanPigRat |
| Predicted Reactivity | Dog: 86%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 93% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GAC GAM GLS AAD20 KGA GLS1 |
|---|---|
| Target | GLS |
| Molecular Weight(MW) | 73 KDa (note) |
Application
WB analysis of ACHN cells using GLS antibody at 0.2-1μg/ml.
Application
WB analysis of rat kidney cortex, kidney proximal tubules from cortex, rat brain tissue, and pig LLCPK1-F cells using GLS antibody at 1:2000. Lane 5 : rat kidney cortex Lane 6 : rat kidney proximal tubules from cortex Lane 7 : pig LLCPK1-F cells Lane 8 : rat brain| Positive Control | ACHN , kidney proximal tubules from cortex , LLCPK1-F+pig kidney proximal tubule , rat brain , rat kidney cortex |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
