Application
Western blot analysis using NR3C1 mouse mAb against Hela cell lysate.Application
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NR3C1 mouse mAb with DAB stainingApplication
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);'Application
Immunofluorescence analysis of PC-2 cells using NR3C1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidinApplication
Flow cytometric analysis of K562 cells using NR3C1 mouse mAb (green) and negative control (purple).| Product Name | Glucocorticoid Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [6E6] to Glucocorticoid ReceptorThis gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011] |
| Immunogen | The immunogen corresponding to human NR3C1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6E6 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GRL GCR GCCR GR |
|---|---|
| Target | Glucocorticoid Receptor |
| Molecular Weight(MW) | 86 kDa (note) |
Application
Western blot analysis using NR3C1 mouse mAb against Hela cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NR3C1 mouse mAb with DAB staining
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);'
Application
Immunofluorescence analysis of PC-2 cells using NR3C1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin
Application
Flow cytometric analysis of K562 cells using NR3C1 mouse mAb (green) and negative control (purple).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
