Application
ICC/IF analysis of HeLa cells using SLC2A4 antibody [3G10A3] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
ELISA analysis of control antigen and antigen using SLC2A4 antibody [3G10A3].Application
FACS analysis of HeLa cells using SLC2A4 antibody [3G10A3] (green) or a negative control (purple).Application
ICC/IF analysis of HepG2 cells using SLC2A4 antibody [3G10A3] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of bladder cancer tissues using SLC2A4 antibody [3G10A3].Application
IHC-P analysis of cardiac muscle tissues using SLC2A4 antibody [3G10A3].Application
WB analysis of lysates from (1) untransfected and (2) SLC2A4 (a.a. 224-353)-hIgGFc transfected HEK293 cells using SLC2A4 antibody [3G10A3].Application
WB analysis of (1) HeLa, (2) NIH3T3, (3) 3T3-L1 and (4) mouse heart tissue lysate using SLC2A4 antibody [3G10A3].Application
WB analysis of human SLC2A4 recombinant protein using SLC2A4 antibody [3G10A3].| Product Name | GLUT4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [3G10A3] to GLUT4This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 224 and 353 of human SLC2A4 expressed in E. Coli. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3G10A3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-P:1:50~1:200 IHC-F:1:100~1:500 ELISA:1:5000~1:20000 FACS:1:200~1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GLUT4 |
|---|---|
| Target | SLC2A4 |
| Molecular Weight(MW) | 54.8 kDa (note) |
Application
ICC/IF analysis of HeLa cells using SLC2A4 antibody [3G10A3] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
ELISA analysis of control antigen and antigen using SLC2A4 antibody [3G10A3].
Application
FACS analysis of HeLa cells using SLC2A4 antibody [3G10A3] (green) or a negative control (purple).
Application
ICC/IF analysis of HepG2 cells using SLC2A4 antibody [3G10A3] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of bladder cancer tissues using SLC2A4 antibody [3G10A3].
Application
IHC-P analysis of cardiac muscle tissues using SLC2A4 antibody [3G10A3].
Application
WB analysis of lysates from (1) untransfected and (2) SLC2A4 (a.a. 224-353)-hIgGFc transfected HEK293 cells using SLC2A4 antibody [3G10A3].
Application
WB analysis of (1) HeLa, (2) NIH3T3, (3) 3T3-L1 and (4) mouse heart tissue lysate using SLC2A4 antibody [3G10A3].
Application
WB analysis of human SLC2A4 recombinant protein using SLC2A4 antibody [3G10A3].| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-P:1:50~1:200 IHC-F:1:100~1:500 ELISA:1:5000~1:20000 FACS:1:200~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
