Application
Glutaminase C antibody [GT1075] detects Glutaminase C protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody [GT1075] diluted at 1:400. Blue: Hoechst 33342 staining.Application
Glutaminase C (GAC) antibody detects Glutaminase C (GAC) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody diluted at a dilution of 1:1000.| Product Name | Glutaminase C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT1075] to Glutaminase C (glutaminase isoform 3)Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase C. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT1075 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KGA GLS GLS1 GAM AAD20 GAC glutaminase |
|---|---|
| Target | Glutaminase C |
| Molecular Weight(MW) | 65 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Glutaminase C antibody [GT1075] detects Glutaminase C protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody [GT1075] diluted at 1:400. Blue: Hoechst 33342 staining.
Application
Glutaminase C (GAC) antibody detects Glutaminase C (GAC) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody diluted at a dilution of 1:1000.| Positive Control | MCF-7 , MDA-MB-231 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
