Application
Glycerol kinase antibody [N3C2], Internal detects Glycerol kinase protein at mitochondria by immunofluorescent analysis. Sample: Huh-7 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Glycerol kinase protein stained by Glycerol kinase antibody [N3C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:500| Product Name | Glycerol kinase antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Glycerol kinase (glycerol kinase)The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glycerol kinase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chimpanzee, BovineMouse (97%), Rat (97%), Xenopus laevis (90%), Pig (97%), Chimpanzee (100%), Bovine (97%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GK1 antibody GKD antibody GK antibody glycerokinase antibody ATP:glycerol 3-phosphotransferase antibody glycerol kinase antibody |
|---|---|
| Target | Glycerol kinase |
| Molecular Weight(MW) | 61 kDa (note) (4) |
| Cellular Localization | Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm |
Application
Glycerol kinase antibody [N3C2], Internal detects Glycerol kinase protein at mitochondria by immunofluorescent analysis. Sample: Huh-7 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Glycerol kinase protein stained by Glycerol kinase antibody [N3C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:500| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
