Application
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12% SDS-PAGE Glyoxalase I antibody [N1C3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using GLO1 antibody at 1:100 dilution.| Product Name | Glyoxalase I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glyoxalase I (glyoxalase I)The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Rhesus Monkey, BovineRat (91%), Xenopus laevis (82%), Rhesus Monkey (97%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GLOD1 antibody GLYI antibody GLO1 antibody aldoketomutase antibody glyoxalase domain containing 1 antibody lactoylglutathione lyase antibody S-D-lactoylglutathione methylglyoxal lyase antibody lactoyl glutathione lyase antibody methylglyoxalase ant |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) |
Application
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12% SDS-PAGE Glyoxalase I antibody [N1C3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using GLO1 antibody at 1:100 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , Mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
