Application
Untreated (–) and treated (+) whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody diluted at 1:1000 (left panel) and 1:500 (right panel).Application
Glypican 1 antibody detects Glypican 1 protein at cell membrane in human pancreas by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas, normal (left) and cancer (right). Glypican 1 antibody diluted at 1:250.| Product Name | Glypican 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Glypican 1 (glypican 1)Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus MonkeyMouse (89%), Rat (90%), Rhesus Monkey (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GPC1 antibody Glypican 1 antibody Glypican 1 antibody glypican antibody |
|---|---|
| Target | Glypican 1 |
| Molecular Weight(MW) | 62 kDa (note) |
| Cellular Localization | Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-1: Secreted , extracellular space |
Application
Untreated (–) and treated (+) whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody diluted at 1:1000 (left panel) and 1:500 (right panel).
Application
Glypican 1 antibody detects Glypican 1 protein at cell membrane in human pancreas by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas, normal (left) and cancer (right). Glypican 1 antibody diluted at 1:250.| Positive Control | A431 , A431 (24 μg/ml Tunicamycin treatment for 16 hr) , A431 (4 mM Benzyl glucosinolate treatment for 32 hr) , A431(24 μg/ml Tunicamycin treatment for 16 hr and 4 mM Benzyl glucosinolate treatment for 32 hr) , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
