Application
ICC/IF analysis of HeLa cells using GPC3 antibody [9C2] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
ELISA analysis of control antigen and antigen using GPC3 antibody [9C2].Application
FACS analysis of Jurkat cells using GPC3 antibody [9C2] (green) or a negative control (red).Application
IHC-P analysis of breast cancer tissues using GPC3 antibody [9C2].Application
IHC-P analysis of liver cancer tissues using GPC3 antibody [9C2].Application
WB analysis of (1) HepG2, (2) HEK293, (3) Jurkat, (4) SK-N-SH, (5) PC-12, (6) F9 and (7) mouse liver lysates using GPC3 antibody [9C2].Application
WB analysis of human GPC3 (a.a. 55-200) recombinant protein using GPC3 antibody [9C2].| Product Name | Glypican-3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [9C2] to GPC3Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] |
| Immunogen | The immunogen corresponding to human GPC3 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9C2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | SDYS DGSX SGB OCI-5 MXR7 SGBS1 SGBS GTR2-2 |
|---|---|
| Target | GPC3 |
| Molecular Weight(MW) | 65.5 kDa (note) |
Application
ICC/IF analysis of HeLa cells using GPC3 antibody [9C2] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
ELISA analysis of control antigen and antigen using GPC3 antibody [9C2].
Application
FACS analysis of Jurkat cells using GPC3 antibody [9C2] (green) or a negative control (red).
Application
IHC-P analysis of breast cancer tissues using GPC3 antibody [9C2].
Application
IHC-P analysis of liver cancer tissues using GPC3 antibody [9C2].
Application
WB analysis of (1) HepG2, (2) HEK293, (3) Jurkat, (4) SK-N-SH, (5) PC-12, (6) F9 and (7) mouse liver lysates using GPC3 antibody [9C2].
Application
WB analysis of human GPC3 (a.a. 55-200) recombinant protein using GPC3 antibody [9C2].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
