Application
GM130 antibody detects GM130 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GM130 protein stained by GM130 antibody diluted at 1:200. Blue: Hoechst 33342 staining.Application
GM130 antibody detects GM130 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GM130 antibody diluted by 1:1000.Application
Immunoprecipitation of GM130 protein from HeLa whole cell extracts using 5 μg of GM130 antibody . Western blot analysis was performed using GM130 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | GM130 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GM130 (golgin A2)The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GM130. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | golgin A2 GOLGA2 |
|---|---|
| Target | GM130 |
| Molecular Weight(MW) | 113 kDa (note) |
| Cellular Localization | Golgi apparatus , Golgi stack membrane; Peripheral membrane protein |
Application
GM130 antibody detects GM130 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GM130 protein stained by GM130 antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Application
GM130 antibody detects GM130 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GM130 antibody diluted by 1:1000.
Application
Immunoprecipitation of GM130 protein from HeLa whole cell extracts using 5 μg of GM130 antibody . Western blot analysis was performed using GM130 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
