WB
WB result of GOLGA2/GM130 antibody (1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells.WB
Various lysates were subjected to SDS PAGE followed by western blot with GOLGA2/GM130 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L), CL594-Phalloidin (red).| Product Name | GOLGA2/GM130 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human GM130;GOLGA2 protein. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:5000-1:50000 ICC/IF:1:500-1:2000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | GOLGA2 |
|---|---|
| Gene Synonyms | GM130 DEDHMB |
| Gene Full Name | golgin A2 |
| Gene Summary | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010] |
| Alternative Names | GM130 GOLGA2 130 kDa cis-Golgi matrix protein 2A4F11 GM130 autoantigen |
| Molecular Weight(MW) | 110kDa(Observed MW:130kDa) |
| Cellular Localization | Cytoplasm,Cytoskeleton,Golgi apparatus,Membrane,Microtubule. |
WB
WB result of GOLGA2/GM130 antibody (1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells.
WB
Various lysates were subjected to SDS PAGE followed by western blot with GOLGA2/GM130 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L), CL594-Phalloidin (red).| Application Notes | WB:1:5000-1:50000 ICC/IF:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
