Recommended Starting Dilutions: ELISA: Max Dilution 0.5 ug/ml. Western Blotting: 0.1 ug/ml - 0.2 ug/ml. Functional assay : The functional activity is based on the antibody's ability to neutralise the activity or murine GM-CSF. To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/ml), a concentration of 0.012-0.020 µg/ml of this antibody is required. Optimal dilution / concentration should be determined by the end user.:
This antibody recognizes mouse granulocyte-macrophage colony-stimulating factor (GM-CSF).
Application Notes
Recommended Starting Dilutions: ELISA: Max Dilution 0.5 ug/ml. Western Blotting: 0.1 ug/ml - 0.2 ug/ml. Functional assay : The functional activity is based on the antibody's ability to neutralise the activity or murine GM-CSF. To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/ml), a concentration of 0.012-0.020 µg/ml of this antibody is required. Optimal dilution / concentration should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Prior to reconstitution store at +4°C. Following reconstitution store at -20°C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.