Application
Immunofluorescence of GOLPH3 in Rat Lung cells with GOLPH3 antibody at 20 ug/mL.Application
Western blot analysis of GOLPH3 in rat lung tissue lysate with GOLPH3 antibody at (A) 0.5 and (B) 1 ug/ml.| Product Name | GOLPH3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GOLPH3GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Golgi phosphoprotein3 Golgi phosphoprotein 3 MIDAS GOLPH3 GPP34 GOPP1 |
|---|---|
| Target | GOLPH3 |
| Tissue Specificity | This antibody is specific for GOLPH3 |
Application
Immunofluorescence of GOLPH3 in Rat Lung cells with GOLPH3 antibody at 20 ug/mL.
Application
Western blot analysis of GOLPH3 in rat lung tissue lysate with GOLPH3 antibody at (A) 0.5 and (B) 1 ug/ml.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
