Application
Immunofluorescence analysis of methanol-fixed HeLa, using GOT2 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GOT2 antibody detects GOT2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 10% SDS-PAGE GOT2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GOT2 antibody detects GOT2 protein by western blot analysis. A. 50 µg rat lung lysate/extract 10% SDS-PAGE GOT2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. GOT2 antibody dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2 antibody at 1:500 dilution.Application
GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GOT2 antibody diluted at 1:500.Application
GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GOT2 antibody diluted at 1:500.| Product Name | GOT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GOT2 (glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2))Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Chicken, BovineXenopus laevis (81%), Dog (92%), Pig (89%), Chicken (82%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ40994 antibody KAT4 antibody KATIV antibody mitAAT antibody GOT2 antibody aspartate aminotransferase mitochondrial antibody fatty acid-binding protein antibody FABPpm antibody glutamate oxaloacetate transaminase 2 antibody kynurenine aminotran |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix , Cell membrane |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using GOT2 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GOT2 antibody detects GOT2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 10% SDS-PAGE GOT2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GOT2 antibody detects GOT2 protein by western blot analysis. A. 50 µg rat lung lysate/extract 10% SDS-PAGE GOT2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. GOT2 antibody dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2 antibody at 1:500 dilution.
Application
GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GOT2 antibody diluted at 1:500.
Application
GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GOT2 antibody diluted at 1:500.| Positive Control | HepG2 , Neuro2A , rat lung |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
