Rabbit polyclonal antibody to GPA33The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008]
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIHC-PWB
P-ELISA:1:500-1000, WB:1:100-500, IHC-P:1:100-500 (based on 0.5 mg/ml):
Species Reactivity
HumanMouseRat
Concentration
0.5mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
glycoprotein A33 (transmembrane) A33
Target
GPA33
Application
Application
IHC-P analysis of human gastric carcinoma tissue using GPA33 antibody at a dilution of 1:200.
Application Notes
P-ELISA:1:500-1000, WB:1:100-500, IHC-P:1:100-500 (based on 0.5 mg/ml):
Additional Information
Form
Liquid
Storage Instructions
Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Storage Buffer
10 mM PBS, pH 7.4 with 10 mg/ml BSA and 0.1% sodium azide