Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GPI antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 B: Raji 7.5% SDS PAGE diluted at 1:10000Application
GPI antibody detects GPI protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: GPI protein stained by GPI antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | GPI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GPI (glucose-6-phosphate isomerase)This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GPI. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Rabbit, Chicken, Chimpanzee, BovineRat (89%), Xenopus laevis (81%), Pig (92%), Rabbit (92%), Chicken (86%), Chimpanzee (99%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AMF antibody, DKFZp686C13233 antibody, GNPI antibody, NLK antibody, PGI antibody, PHI antibody, SA-36 antibody, SA36 antibody, GPI antibody, phosphosaccharomutase antibody, neuroleukin antibody, autocrine motility factor antibody, sperm antigen 36 antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) (8) |
| Cellular Localization | Cytoplasm , Secreted |
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GPI antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Raji 7.5% SDS PAGE diluted at 1:10000
Application
GPI antibody detects GPI protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: GPI protein stained by GPI antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | H1299 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
