Application
Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using GPNMB antibody at 1:500 dilution.| Product Name | GPNMB antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GPNMB (glycoprotein (transmembrane) nmb)The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GPNMB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HGFIN antibody NMB antibody GPNMB antibody osteoactivin antibody glycoprotein NMB antibody transmembrane glycoprotein NMB antibody transmembrane glycoprotein HGFIN antibody glycoprotein nmb-like protein antibody glycoprotein (transmembrane) nmb ant |
|---|---|
| Molecular Weight(MW) | 64 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential) , Melanosome |
Application
Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using GPNMB antibody at 1:500 dilution.| Positive Control | PC-3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
