Western blot analysis of GPR15 in human heart lysate with anti- GPR15 (NT) in the absence (lane 1) or presence of specific peptide (lane 2) or non related peptide (lane 3)
Rabbit polyclonal to GPR15Human immunodeficiency virus (HIV) and related viruses require co-receptors to infect target cells. Some G -protein coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 andV28 were recently identified as HIV co-receptors. The genes encoding human and monkey GPR15/BOB (for G-protein-coupled receptor 15 and brother of Bonzo, respectively,) were recently cloned (1,2). This novel G protein-coupled receptor serves as co-receptor for simian immunodeficiency virus (SIV), and for strains of HIV-2and M-tropic HIV-1 (2,3). The ligand for GPR15 has not been identified yet.
Immunogen
Synthetic Peptide - amino acids 13-28 of human GPR15. The sequence differs from those of African green monkey and pig-tailed macaque BOB by one amino acid
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
For WB: Use at a dilution of 1:500-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouseRat
Concentration
0.5mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
GPR15, 2838, P49685, 601166, MGC126828, MGC126830
Target
GPR15
Application
Application
Western blot analysis of GPR15 in human heart lysate with anti- GPR15 (NT) in the absence (lane 1) or presence of specific peptide (lane 2) or non related peptide (lane 3)
Positive Control
Jurkat whole cell lysate, A549 whole cell lysate.
Application Notes
For WB: Use at a dilution of 1:500-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.