Application
Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30 antibody at 1:150 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (40ug of whole cell lysate) A: RL952 cell B: GPR30 shRNA-transfected RL952 cell diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GPR30 antibody [C2C3], C-term detects GPR30 protein by western blot analysis. A. 50 µg 293T whole cell lysate/extract B. 50 µg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling) C. 50 µg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling) 5% SDS-PAGE GPR30 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GPR30 antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GPR30 (G protein-coupled estrogen receptor 1)This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPR30. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | RatRat (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CEPR antibody, CMKRL2 antibody, DRY12 antibody, FEG-1 antibody, GPCR-Br antibody, GPR30 antibody, LERGU antibody, LERGU2 antibody, LyGPR antibody, MGC99678 antibody, GPER antibody, mER antibody, IL8-related receptor DRY12 antibody, lymphocyte-derived G-pro |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (3) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein , Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity) , Golgi apparatus membrane; Multi-pass membrane protein (By similarity) |
Application
Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30 antibody at 1:150 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (40ug of whole cell lysate) A: RL952 cell B: GPR30 shRNA-transfected RL952 cell diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GPR30 antibody [C2C3], C-term detects GPR30 protein by western blot analysis. A. 50 µg 293T whole cell lysate/extract B. 50 µg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling) C. 50 µg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling) 5% SDS-PAGE GPR30 antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , RL952 cell , GFP-human GPR30-transfected 293T (No boiling) , GFP-human GPR30 and GPR30 siRNA-transfected 293T (No boiling) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
