Application
Western blot analysis of CRTH2 in CRTH2-transfected Jurkat cell lysate. Does not detect CRTH2 in non-transfected parental Jurkat cells.| Product Name | GPR44 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal to GPR44 ( C terminus )CRTH2 (chemoattractant receptor-homologous molecule), which is also known as the putative G protein-coupled receptor (GPR44), is expressed on Th2 cells and is a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells. CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Q9Y5Y4, CRTH2, GPR44, 11251, 604837, CD294, DL1R, DP2 |
|---|---|
| Target | GPR44 |
| Tissue Specificity | Reacts with residues 329-348 [DDSELGGAGSSRRRRTSSTA] of the CRTH2 (GPR44) protein. This sequence is near the C terminus. Cross reacts with rat and mouse. |
Application
Western blot analysis of CRTH2 in CRTH2-transfected Jurkat cell lysate. Does not detect CRTH2 in non-transfected parental Jurkat cells.| Application Notes | Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
