Application
Western blot analysis of GPR75 expression in HEK293T (A); A549 (B); SP2/0 (C); rat kidney (D) whole cell lysates.Application
Immunofluorescent analysis of GPR75 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Product Name | GPR75 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GPR75GPR75 is a member of the G protein-coupled receptor family. GPRs are cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand.[supplied by OMIM, Jul 2002] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR75. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | WI31133 , GPRchr2 , G protein-coupled receptor 75 |
|---|---|
| Target | GPR75 |
| Tissue Specificity | Recognizes endogenous levels of GPR75 protein. |
Application
Western blot analysis of GPR75 expression in HEK293T (A); A549 (B); SP2/0 (C); rat kidney (D) whole cell lysates.
Application
Immunofluorescent analysis of GPR75 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Application Notes | WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
