Application
GPS2 antibody detects GPS2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPS2 protein stained by GPS2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPS2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:500| Product Name | GPS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GPS2 (G protein pathway suppressor 2)This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GPS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (98%), Rat (98%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AMF-1 antibody, MGC104294 antibody, MGC119287 antibody, MGC119288 antibody, MGC119289 antibody, GPS2 antibody, GPS-2 antibody, G protein pathway suppressor 2 antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (2) |
Application
GPS2 antibody detects GPS2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPS2 protein stained by GPS2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPS2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:500| Positive Control | A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
