Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GPX1 antibody at 1:100 dilution.Application
GPX1 antibody detects GPX1 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE GPX1 antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GPX1 antibody detects GPX1 protein by western blot analysis. A.50 µg rat liver lysate/extract 12% SDS-PAGE GPX1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Glutathione peroxidase 1 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GPX1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GPX1 (glutathione peroxidase 1)This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSHPX1 antibody, MGC14399 antibody, MGC88245 antibody, GPX1 antibody, glutathione peroxidase 1 antibody, GSHPx-1 antibody, cellular glutathione peroxidase antibody, GPx-1 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using GPX1 antibody at 1:100 dilution.
Application
GPX1 antibody detects GPX1 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE GPX1 antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GPX1 antibody detects GPX1 protein by western blot analysis. A.50 µg rat liver lysate/extract 12% SDS-PAGE GPX1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Glutathione peroxidase 1 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Molt-4 , Raji , mouse liver , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
