Application
Sample (30 µg of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 μg of GPX2 antibody [C1C3] . Western blot analysis was performed using GPX2 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPX2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using GPX2 antibody at 1:500 dilution.| Product Name | GPX2 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GPX2 (glutathione peroxidase 2 (gastrointestinal))This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rabbit, Rhesus Monkey, Chimpanzee, BovineRat (100%), Dog (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GI-GPx antibody, GPRP antibody, GSHPX-GI antibody, GSHPx-2 antibody, GPX2 antibody, GPx-2 antibody, GPRP-2 antibody, glutathione peroxidase-related protein 2 antibody, GPx-GI antibody, glutathione peroxidase 2 antibody, gastrointestinal glutathione peroxid |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 μg of GPX2 antibody [C1C3] . Western blot analysis was performed using GPX2 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPX2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using GPX2 antibody at 1:500 dilution.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
