Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000| Product Name | GRAF antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GRAF (Rho GTPase activating protein 26)Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GRAF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | ChickenChicken (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ42530 antibody, GRAF antibody, KIAA0621 antibody, OPHN1L antibody, OPHN1L1 antibody, ARHGAP26 antibody, GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody, oligophrenin-1-like protein antibody, rho GTPase-activating protein 26 an |
|---|---|
| Molecular Weight(MW) | 92 kDa (note) (2) |
| Cellular Localization | Cell junction , focal adhesion (By similarity) , Cytoplasm , cytoskeleton (By similarity) |
Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
