Application
Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:2000Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:3000Application
GRAP antibody [N1C3] detects GRAP protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GRAP antibody [N1C3] dilution: 1:500.| Product Name | GRAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GRAP (GRB2-related adaptor protein)This gene encodes a member of the GRB2/Sem5/Drk family. This member functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GRAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, Rhesus Monkey, BovineRat (93%), Chicken (80%), Rhesus Monkey (98%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC64880 antibody GRAP antibody growth factor receptor-bound protein 2-related adaptor protein antibody GRB2-related adapter protein antibody GRB2-related adaptor protein antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:2000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:3000
Application
GRAP antibody [N1C3] detects GRAP protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GRAP antibody [N1C3] dilution: 1:500.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
