| Product Name | GRASP65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit anti-GRASP65 polyclonal antibodyGRASP65 (Golgi ReAssembly Stacking Protein) is a conserved protein that participates in the stacking of cisternae. This protein associates with GM130 and is phosphorylated by the MEK1 substrate ERK2, Polo-like kinase (Plk) and cdc2-cyclin B. GRASP65 thus plays a role in a cell cycle-dependent reorganization of the Golgi apparatus and may control mammalian cell entry into mitosis. |
| Immunogen | Rabbits were immunized with recombinant (6His-tagged) mouse GRASP65 protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| IF (1:500 – 1:1,000), IP assay dependent, WB (1:5,000 – 1:10,000) Predicted molecular weight: 46 kDa | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 64689, GRASP65, GORASP1, Q9BQQ3, P65, GOLPH5, FLJ23443, 606867, MGC118894, MGC118897 |
|---|---|
| Target | GRASP65 |
| Cellular Localization | Cis-Golgi; peripherical membrane protein tighly associated with the cytoplasmic face of the membrane. Undergoes rapid exchange with the cytosol. |
| Application Notes | IF (1:500 – 1:1,000), IP assay dependent, WB (1:5,000 – 1:10,000) Predicted molecular weight: 46 kDa |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20º C. Avoid multiple freeze/thaw cycles. |
| Storage Buffer | Whole serum contianing 50% glycerol & 0.01% thimerosal. |
