Application
GRB2 antibody detects GRB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GRB2 antibody dilution: 1:500.Application
GRB2 antibody detects GRB2 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. GRB2 antibody dilution: 1:500.Application
GRB2 antibody detects GRB2 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. GRB2 antibody dilution: 1:500.Application
GRB2 antibody detects GRB2 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GRB2 antibody dilution: 1:500.Application
GRB2 antibody detects GRB2 protein at nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. GRB2 antibody dilution: 1:500.Application
GRB2 antibody detects GRB2 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. GRB2 antibody dilution: 1:500.Application
GRB2 antibody detects GRB2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GRB2 protein stained by GRB2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE GRB2 antibody diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 12% SDS PAGE diluted at 1:1000| Product Name | GRB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GRB2 (growth factor receptor-bound protein 2)The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRB2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Chimpanzee, BovinePig (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ASH antibody, EGFRBP-GRB2 antibody, Grb3-3 antibody, MST084 antibody, MSTP084 antibody, NCKAP2 antibody, GRB2 antibody, protein Ash antibody, growth factor receptor-bound protein 3 antibody, SH2/SH3 adapter GRB2 antibody, abundant SRC homology antibody, gr |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (2) |
| Cellular Localization | Golgi apparatus |
Application
GRB2 antibody detects GRB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GRB2 antibody dilution: 1:500.
Application
GRB2 antibody detects GRB2 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. GRB2 antibody dilution: 1:500.
Application
GRB2 antibody detects GRB2 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. GRB2 antibody dilution: 1:500.
Application
GRB2 antibody detects GRB2 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GRB2 antibody dilution: 1:500.
Application
GRB2 antibody detects GRB2 protein at nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. GRB2 antibody dilution: 1:500.
Application
GRB2 antibody detects GRB2 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. GRB2 antibody dilution: 1:500.
Application
GRB2 antibody detects GRB2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GRB2 protein stained by GRB2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE GRB2 antibody diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 , JC , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
