Application
Western blot analysis of GRF-1 phosphorylation expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Product Name | GRF 1 (phospho Tyr1105) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GRF 1 (phospho Tyr1105)The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide derived from human GRF 1 around the phosphorylation site of Tyr1105 |
| Modification | p-Tyr1105 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | phospho GRF 1 (phospho Tyr1105) (pY1105) GRF 1 (phospho Tyr1105) Phospho Y1105 GRF 1 (phospho Tyr1105) Phospho Tyr1105 Phospho GRF 1 (phospho Tyr1105) Y1105 GRF 1 (phospho Tyr1105) Phospho GRF 1 (phospho T |
|---|---|
| Target | GRF 1 (phospho Tyr1105) |
| Tissue Specificity | GRLF1 |
Application
Western blot analysis of GRF-1 phosphorylation expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
