Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GRHPR monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR.Application
HEK293T cells transfected with either RC200963 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GRHPR antibody, and then analyzed by flow cytometry.| Product Name | GRHPR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4H2] to GRHPREnzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate. |
| Immunogen | Full length human recombinant protein of human GRHPR (NP_036335) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4H2 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| Flow Cytometry 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GLXR |
|---|---|
| Target | GRHPR |
| Tissue Specificity | is specific for human GRHPR (NP_036335). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GRHPR monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR.
Application
HEK293T cells transfected with either RC200963 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GRHPR antibody, and then analyzed by flow cytometry.| Application Notes | Flow Cytometry 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
