Application
WB analysis of recombinant hGH and 20kDa hGH using Growth Hormone antibody [g3H5] at a dilution of 1:500.| Product Name | Growth Hormone antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [g3H5] to Growth HormoneThe protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human growth hormone (27-217aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | g3H5 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | growth hormone 1 IGHD1B GH-N GH hGH-N GHN GH1 |
|---|---|
| Target | Growth Hormone |
| Light Chain Type | kappa |
Application
WB analysis of recombinant hGH and 20kDa hGH using Growth Hormone antibody [g3H5] at a dilution of 1:500.| Application Notes | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
