Application
GRP75 antibody detects GRP75 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody diluted at a dilution of 1:2500.Application
Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 μg of GRP75 antibody . Western blot analysis was performed using GRP75 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Whole cell extract (6 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody diluted at 1:500.| Product Name | Grp75 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Grp75 (heat shock 70kDa protein 9 (mortalin))The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Grp75. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMousePig |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, BovineRat (99%), Zebrafish (87%), Xenopus laevis (94%), Chicken (95%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CSA antibody GRP-75 antibody GRP75 antibody HSPA9B antibody MGC4500 antibody MOT antibody MOT2 antibody MTHSP75 antibody PBP74 antibody HSPA9 antibody heat shock 70kD protein 9B antibody 75 kDa glucose-regulated protein antibody peptide-binding |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data. |
| Cellular Localization | Mitochondrion |
Application
GRP75 antibody detects GRP75 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody diluted at a dilution of 1:2500.
Application
Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 μg of GRP75 antibody . Western blot analysis was performed using GRP75 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Whole cell extract (6 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody diluted at 1:500.| Positive Control | SCC-15 , 293T , A431 , HeLa , HepG2 , *IPEC-J2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
