Application
WB analysis of untreated (Lane 1) and anisomycin-treated (Lane 2, at a concentartion of 500 ng/ml for 30min) HT29 cell extracts using GRP94 (phospho Ser254) antibody.| Product Name | GRP94 (phospho Ser254) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HSP90B (phospho S254) |
| Modification | p-Ser254 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | HSP90B (phospho S254) |
|---|
Application
WB analysis of untreated (Lane 1) and anisomycin-treated (Lane 2, at a concentartion of 500 ng/ml for 30min) HT29 cell extracts using GRP94 (phospho Ser254) antibody.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS ( without Mg2+ and Ca2+ ), pH 7. 4 , 150mM NaCl , 0.02% sodium azide and 50% glycerol . |
