Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GSDMD antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.| Product Name | GSDMD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSDMD (gasdermin D)Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GSDMD. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DF5L antibody DFNA5L antibody FLJ12150 antibody GSDMDC1 antibody GSDMD antibody gasdermin-D antibody gasdermin domain containing 1 antibody gasdermin domain-containing protein 1 antibody gasdermin D antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (3) |
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GSDMD antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
