Application
Flow cytometric analysis of Hela cells using GSK3B mouse mAb (green) and negative control (purple)Application
Western blot analysis using GSK3B mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.Application
Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mAb with DAB staining.| Product Name | GSK3 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3D10] to GSK3 betaThe protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3D10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Gene Synonyms | GSK3B |
|---|---|
| Target | GSK3B |
| Molecular Weight(MW) | 46 kDa (note) |
Application
Flow cytometric analysis of Hela cells using GSK3B mouse mAb (green) and negative control (purple)
Application
Western blot analysis using GSK3B mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.
Application
Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mAb with DAB staining.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
